tag:blogger.com,1999:blog-5350635493235522700.post1351567080084350146..comments2009-06-15T07:09:50.338-04:00Lab Boyhttp://www.blogger.com/profile/05642702084948743337noreply@blogger.comBlogger8125tag:blogger.com,1999:blog-5350635493235522700.post-34695231934834405292009-06-15T07:09:50.338-04:002009-06-15T07:09:50.338-04:00Ah, the classics.Lab Boyhttp://www.blogger.com/profile/05642702084948743337noreply@blogger.comtag:blogger.com,1999:blog-5350635493235522700.post-60898596184908349892009-06-14T21:16:40.048-04:002009-06-14T21:16:40.048-04:00I just swear at the bugs and threaten them.spurgehttp://www.blogger.com/profile/08448877864223502410noreply@blogger.comtag:blogger.com,1999:blog-5350635493235522700.post-18422570366693711042009-06-13T12:47:35.850-04:002009-06-13T12:47:35.850-04:00Yikes. <br />Glad I don&#39;t have to use bioreactors.<br />Just good old-fashioned tubes and plates for me, thanks.<br />Did you remember to tell whoever did the work to stand on one leg while working? Maybe a chicken sacrifice would help.Lab Boyhttp://www.blogger.com/profile/05642702084948743337noreply@blogger.comtag:blogger.com,1999:blog-5350635493235522700.post-11873120524872325712009-06-11T21:15:33.289-04:002009-06-11T21:15:33.289-04:00So far we have been spared any DNA/RNA contamination.<br /><br /><br />It happened again today. I am glad I am not running the fermenter.<br /><br />That is what we get when we put two bioreactors on the same bench using different organisms.spurgehttp://www.blogger.com/profile/08448877864223502410noreply@blogger.comtag:blogger.com,1999:blog-5350635493235522700.post-43174690927296844962009-06-05T21:24:50.055-04:002009-06-05T21:24:50.055-04:00AARGH!!! That I understand. I hate contamination issues! Bacteria are frustrating enough on their own, but try to de-contaminate a lab infested with a 189 bp amplicon that has been sprayed around by a clueless intern! For a real-time PCR assay! AARGHHH!<br /><br />No, using the laminar flow hood with an amplified product to set up your gel is not cool!Lab Boyhttp://www.blogger.com/profile/05642702084948743337noreply@blogger.comtag:blogger.com,1999:blog-5350635493235522700.post-67648217931985720912009-06-04T21:02:01.285-04:002009-06-04T21:02:01.285-04:00We recently had some contamination issues in the lab so it was on my mind.<br /><br />A friend of mine has some Indian starter Lactobacillus I may try.spurgehttp://www.blogger.com/profile/08448877864223502410noreply@blogger.comtag:blogger.com,1999:blog-5350635493235522700.post-25582303408929537442009-06-04T19:59:30.240-04:002009-06-04T19:59:30.240-04:00I haven&#39;t sanitized (sterilize is virtually impossible in the standard kitchen) the containers in the past. It would be a good idea as a precautionary measure, but I do wash them in very hot water before I use them. The <i>Lactobacillus</i> will outcompete most things that settle out of the air.<br /><br />The point of going to 190 F is basically to re-pasteurize the milk (assuming it was in the first place, dunno where you get your milk).<br /><br />I&#39;ll add a few notes to the post to clarify that. Thanks for asking. I end up doing so much of the micro stuff automatically that I forget to think about it when writing it up.Lab Boyhttp://www.blogger.com/profile/05642702084948743337noreply@blogger.comtag:blogger.com,1999:blog-5350635493235522700.post-1766916037458618952009-06-04T09:33:16.668-04:002009-06-04T09:33:16.668-04:00Great post. I may have to give that a try. Even if it seems allot like what I do at work.<br /><br />Do the Lactobacillus out compete any potential contaminating organisms?<br /><br />Do you sterilize your containers?spurgehttp://www.blogger.com/profile/08448877864223502410noreply@blogger.com